Sentence examples for buffers were made by from inspiring English sources

Likewise, phosphate buffers were made by combining 10 mM lithium ion solutions of lithium monobasic and lithium dibasic phosphate to the desired pH.

Tris buffer was made from Tris base and adjusted to the correct pH with 5 M HCl; MOPS and MES buffers were made by adjusting the pH using 5 M NaOH.

The buffers were made by mixing two aliquots of the aforementioned buffer set to either pH 4.0 (buffer A) or 8.0 (buffer B) in 10% increments, starting from 0% B to 100% B, giving 11 points total between pH 4.0 and 8.0.

A "control buffer" was made by supplementing XB-CSF with the energy mix, protease inhibitors, nocodazole, and cytochalasin B. PCM recruitment was preformed according to the methods of Murray and Kirschner46 with modifications from Simerly et al34.

This buffer is made by adding NaN3 at a final concentration of 0.01% to PBS/BSA.

Qualitative results are summarized in Table 2. Comparisons of binding in 140Na/80HEPES/10Mg and 135K/25Na/50HEPES/1MNa buffers to that in 135K/25Na/50HEPES/10Mg buffer are made by taking the differences of their normalized intensities according to Buffer X denotes 140Na/80HEPES/10Mg or 135K/25Na/50HEPES/1MNa.

TE-buffer was made by mixing a 1 M solution of Tris with a 0.1 M EDTA (pH 8) solution to give concentrations of 5 mM Tris and 10 mM EDTA adjusting the pH to 7.2 using HCl.

The lysis buffer was made by combining 20 ml of PBS, 1nM of EDTA, 5 mM NaF, 6 M Urea, 0.1 ml of Triton ×100, and 2 packets on Halt protease/phosphatase inhibitors from Thermo Scientific (Waltham, MA, USA).

All buffers were made in RNase-free water.

After addition of 30 µl of 4× SDS sample buffer, lysates were made by boiling for 10 min, freezing on dry ice for 20 min, and boiling for 10 min.

Injections of 100 nl (N = 2), 20 nl (N = 2), or 5 nl (N = 6) of 5% biocytin (SIGMA-Aldrich Chemicals, Germany), dissolved in 0.05 M TRIS buffer (pH 7.6), were made by pressure (one injection per animal) over a period of two minutes using fine glass micropipettes (tip diameter 20 µm) and an oil hydraulic nanoliter delivery system (WPI, Germany) (Table 1).