Sentence examples for buffered with acetate from inspiring English sources

Briefly, 0.1 300 μg/ml BNO 1095 was added to 100 μl ethanol (blank controls) or to 100 μl of a solution of the stable free radical in ethanol buffered with acetate to pH 5.5 (50 μM, corresponding to 19.7 μg/ml), in a 96-well plate.

Accordingly, the Seplyte solutions are buffered with acetate and gluconate, rather than lactate, to minimize the lactate load during organ dysfunction, while maintaining pH buffering and energy production capabilities through metabolism of acetate into bicarbonate [32] and metabolism of gluconate to produce ATP [33], respectively.

It was applied to rough nickel electrodes in aqueous solutions of 10−2 M quinhydrone and 0.5 M K2SO4, buffered with acetic acid and sodium acetate.

In the case of the control PET/CT imaging with "free Cu", 50 μl CuCl2 solution buffered with sodium acetate (containing 5 MBq radioactivity) was injected intravenously via the tail vein.

To analyze urinary 1-OHP and 2-NAPH, urine samples were buffered with sodium acetate (pH 5.0) and hydrolyzed enzymatically using β-glucuronidase with sulfatase activity (Sigma, St . Louis MO, USA) for 16 hr at 37°C in a shaking water bath (Jongeneelen et al. 1987; Kim et al. 1999).

Eluates were immediately buffered with 1 M acetate buffer pH 4.75 and extensively dialysed against 10 mM acetate buffer pH 4.75 containing 1 mM ETDA.

The enamel was then covered with standard "o -shaped wax so -shapedxpose a standard round waxdow area (6.83 mm [ 2]) and acetic acid buffered with 0.34 M sodium asetotexpose= 4) wastandards a demineroundation buffer.

Pigments were extracted using 98% methanol buffered with 0.5 M ammonium acetate and analysed by HPLC-DAD according to a protocol modified from Ensminger et al.14.14

The standards and HPLC mobile phases were prepared using Milli-Q water and HPLC grade methanol (Fisher Scientific, Ottawa, ON) and buffered with 10 mM ammonium acetate (Sigma Aldrich, St . Louis MO) or 90% formic acid (Fisher Scientific).

tRNAs were fractionated at 4°C in 7.5% acrylamidde-8 M urea (30 cm long, 0.8 mm thick), buffered with 0.1 M sodium acetate pH 5.0.

The proteins were diluted ∼20 fold to 15 µg/ml into 70% glycerol buffered with 0.2 M ammonium acetate (pH 7.3) and sprayed on freshly cleaved mica before transfer to the rotary stage of an Edward evaporator.