Sentence examples similar to buffered solution were added from inspiring English sources

Briefly, 80 μl of sample solution were added into each well of a 96-well microplate, and then a mixture of 100 μl phosphate substrate solution (p-nitrophenyl phosphate) and 20 μl 1.5 M alkali buffer solution were added to each well.

Glucose (5 mM) in pH 5.0 citrate–Na2HPO4 buffered solutions was added into each well and incubated at 35 °C for 10 min. To each well was added the mixture of 5 nm AuNP seeds (8.3 nM) and AuCl4– (0.6 mM).

A further 20 mL of sodium acetate-acetic acid buffer solution were added to achieve pH 5.3, before dilution to 100 mL with water.

EDC of 50 ml and a Na2CO3-NaHCO3 (1:9) buffer solution were added to adjust the pH of the system to approximately 9.

Weighed brain tissues were homogenized with BHT-Methanol (Sigma, St . Louis MO) and two volumes of chloroform (J.T. Baker, Phillipsburg, NJ) and 0.5 volume of NaH2PO4 (0.2 M -buffer solution were added to the resulting hoM -buffer

In the next step, 115 μl of the buffer solution were added.

For this reason, 0.01 M of CuCl2 solution, 7.5 mM of ethanol neocuproine solution, and 1.0 M of CH3COONH4 buffer solution were added to each test tube, respectively.

After 18 h, 80 μl of hydrated Protein A agarose beads (in a 1 1 slurry in 1× phosphate-buffered solution) were added to each sample and returned to continuous rotation for a further 4 h.

After 15 min, 100 µl of buffer solution was added and mixed.

After being separated with a NdFeB magnet, 50 nmol of streptavidin in a phosphate buffer solution was added.

250 μL of ALP substrate solution (5 mM pNPP diluted in alkaline buffer solution) was added and the plates incubated for 30 min at 37°C.