Sentence examples for buffered solution was added from inspiring English sources

A chromogenic substrate solution (Mg2+ phenolphthalein monophosphate buffered solution) was added to each well.

Glucose (5 mM) in pH 5.0 citrate–Na2HPO4 buffered solutions was added into each well and incubated at 35 °C for 10 min. To each well was added the mixture of 5 nm AuNP seeds (8.3 nM) and AuCl4– (0.6 mM).

Briefly, 80 μl of sample solution were added into each well of a 96-well microplate, and then a mixture of 100 μl phosphate substrate solution (p-nitrophenyl phosphate) and 20 μl 1.5 M alkali buffer solution were added to each well.

After 15 min, 100 µl of buffer solution was added and mixed.

After being separated with a NdFeB magnet, 50 nmol of streptavidin in a phosphate buffer solution was added.

250 μL of ALP substrate solution (5 mM pNPP diluted in alkaline buffer solution) was added and the plates incubated for 30 min at 37°C.

To determine exchangeable Ca and Mg, 10 ml of the extract was transferred to an Erlenmeyer flask and 5 ml of an ammonium chloride and ammonium hydroxide buffer solution was added, followed by addition of 1 ml triethanolamine.

10 mL of phosphate buffer solution was added to the settled sludge and shake to disperse the sludge evenly for 10 min, and then the mixed suspension was centrifuged at 4000 rpm for 10 min.

At predetermined time intervals, 5 mL aliquots of the release medium were withdrawn from the release medium, and the same volume of fresh buffer solution was added to continue the release test.

Ringer buffer solution was added (3 mL buffer per g tissue) and the compound was extracted using adaptive focused acoustic ultrasound equipment (Covaris E210, Covaris Inc).

An equivalent volume (50 μl) of buffer solution was added to the blank or control in place of the extract.