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Catalase (5 μg/mL final concentration) is also added to the above buffered solution to remove the hydrogen peroxide produced by the glucose oxidase.
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Finally, they were washed with buffer solution to remove the unbound protein and excess GA.
The obtained surfaces were washed with buffer solution to remove unbound zeolites before enzyme immobilization.
RNA was washed with buffer solution to remove impurities and eluted in a final volume of 15 μl.
The methanogenic sludge was taken after being acclimated for more than 50 days and rinsed with anaerobic preheated (55°C) buffer solution to remove the residual carbon.
The sediments were centrifuged and rinsed alternately three times with 0.1 M, pH 7.0 phosphate buffer solution to remove non-specifically adsorbed enzymes.
This was predialised overnight against pure buffer solution to remove sugars and other low molecular weight impurities.
Blood was washed three times with Alserver's buffer solution and one time with bicine-saline buffer solution to remove plasma.
Finally, the -S-C6-ssDNA-10b and -S-PEG6 functionalized nanoprisms were rinsed with adequate amount of PBS buffer solution to remove nonspecifically bound thiols.
Cells were seeded in 96-well plates and after adhesion, cultures were washed three times with phosphate buffer solution to remove non-adherent cells.
The specimens were flash frozen in liquid nitrogen at the clinic and shipped to the laboratory in dry ice, thawed on ice, washed in sterile phosphate buffer solution to remove blood, refrozen, and stored at −80°C until sample analysis.
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