Sentence examples for buffered salt solution with from inspiring English sources

Briefly, brain tissue was dissected and dounce homogenized in Hank's buffered salt solution with 20 mM HEPES at pH = 7.4.

This was followed by perfusion with 0.05% collagenase (C5138; Sigma-Aldrich) in Hanks' buffered salt solution with Ca2+ and Mg2+ (Sigma-Aldrich) for 3 5 min.

After 24 and 48 h of incubation, we washed cells in PBS, and suspended 1 × 10 cells per ml in Hank's buffered salt solution with 50 n M tetramethylrhodamine methyl ester (TMRM) (Invitrogen).

The maximum score for each parameter was 4. The colons were excised, placed in calcium/magnesium-free Hank's buffered salt solution with 1% FBS (Gibco, Grand Island, NY, USA) and cleaned of the contents.

Cells were loaded with 3 µM TMRM (Sigma, T5428) in Hank's buffered salt solution (HBSS with Ca2+, Sigma, H8264) for 15 min at 37°C.

Hind feet were dissected and immersed in transfer buffer (Hank's buffered salt solution supplemented with 10 mM HEPES, pH 7.4).

The coverslips were washed 4 times with Hank's buffered salt solution and fixed with freshly made fixative (methanol-glacial acetic acid, 3 1, v/v) for 5 min, followed by air-drying and 15 min Giemsa stain.

Epithelial cells were removed by incubating intestinal tissues in Hank's buffered salt solution (HBSS) supplemented with EDTA and DTT, followed by extensive washing with PBS.

Briefly, mouse renal cortices were dissected and minced in ice-cold dissection solution of Hank's buffered salt solution (HBSS) (Thermo Scientific) supplemented with 10 mM glucose, 5 mM glycine, 1 mM alanine and 15 mM HEPES pH 7.4.

Twenty-four hours after plating, cells were collected, washed in PBS 1× and then suspended in phenol-red free Hank's buffered salt solution (HBSS) 1× supplemented with 10 m m HEPES, 2 μ m Ciclosporin H Alexis BiochemicalsSan Diego, CA CA, USA) and 20 n m TMRM and incubated for 30 min at 37°C.

The slides were then washed with fresh phosphate buffered salt solution (PBS) at room temperature, the cell monolayers fixed with 2.5% glutaraldehyde and routinely processed to microscopy analysis as follows.