Sentence examples for buffered salt solution from inspiring English sources

Hind feet were dissected and immersed in transfer buffer (Hank's buffered salt solution supplemented with 10 mM HEPES, pH 7.4).

Briefly, brain tissue was dissected and dounce homogenized in Hank's buffered salt solution with 20 mM HEPES at pH = 7.4.

The slides were then washed with fresh phosphate buffered salt solution (PBS) at room temperature, the cell monolayers fixed with 2.5% glutaraldehyde and routinely processed to microscopy analysis as follows.

When the cells approximately covered all the bottom of the tissue culture flask, Hanks buffered salt solution (HBSS, pH7.4) was used to wash the cells three times.

Hank's buffered salt solution (HBSS) without CaCl2 and MgCl2.

Hank's buffered salt solution (HBSS) was from Applichem, Darmstadt, Germany.

Briefly, cells were bathed at RT in a HEPES-buffered salt solution (HBSS) containing (mM): 156 NaCl, 3 KCl, 2MgSO4, 1.25 KH2PO4, 2 CaCl2, 10 glucose and 10 HEPES, pH 7.35.

containing a Hepes-buffered salt solution.

Then, cells were rinsed with phosphate-buffered salt solution and air-dried at room temperature for 1 h.

Cells were loaded with the membrane potential-sensitive dye tetramethylrhodamine ethyl ester perchlorate (TMRE; 20 nM in Hepes-buffered salt solution; Invitrogen, Carlsbad, CA, USA).

The addition of small amounts of oxygen to dense cell suspensions incubated anoxically in non-buffered salt solution caused a transient decrease of the extracellular pH (Fig. 2).