Sentence examples for buffered saline solution with from inspiring English sources

The vitreous body was removed and the eyecup was incubated for 30 minutes at 37°C in Hank's Buffered Saline Solution with calcium and magnesium to facilitate separation of the retina from the retinal pigment epithelium (RPE).

Reduced glutathione (GSH) was determined by incubating live cells in Hank's buffered saline solution with 40 µM monochlorobimane (mCB) for 1 h (Rice et al., 1986).

For experiments involving T cell culture with the system L blocker 2-amino-2-norbornanecarboxylic acid (BCH, Sigma), cells were cultured in 50% RPMI culture medium and 50% Hanks' buffered saline solution with or without 50 mΜ BCH.

Using fine scissors, brains were minced into small pieces and incubated for 1 h at 37°C in suspension consisting of one part PBS and one part Hank's buffered saline solution with 25 mmol/L HEPES buffer, pH 7.2 containing 0.25% trypsin (Invitrogen, Waltham, MA, USA), and 40  μg/mL DNase (Sigma-Aldrich, St . Louis MO, USA).

Separated proteins were then transferred to a nitrocellulose membrane using Trans-Blot Turbo transfer system (Bio-Rad) at 25 V for 7 min. After successful transfer, membranes were blocked in 0.5 % dry milk in Tris Buffered saline solution with 1 % Tween (TBST) using SNAP i.d. protein detection system.

A repeatability test using a PBS (phosphate buffered saline) solution with known properties was carried out ten times; the maximum standard deviation was approximately 7% in electrical impedance.

The cells were grown in modified Eagle medium (MEM) with Earle's buffered saline solution supplemented with inactivated bovine foetal serum (100 μl/ml), sodium bicarbonate (1.12 mM), L-glutamin (4 mM) and antibiotics (penicillin: 100 I.E./ml; streptomycin: 100 μg/ml and amphotericin B: 250 ng/ml) (cells and medium delivered by Bio-Whittaker, Walkersville, MD, USA).

16 24 h later, culture medium was aspirated, and cells were washed once with 100 μl assay buffer (Hank's Buffered Saline Solution supplemented with 20 mM HEPES, 1 mM CaCl2 and 1 mM MgCl2, pH 7.4).

The antibodies were dissolved and all washing steps carried in phenol-free, Ca+/Mg+ free, PIPES buffered saline solution, supplemented with 20 mM glucose, 10% human serum.

The cells were resuspended in Hank's buffered saline solution (HBSS) with 0.2% bovine serum albumin (BSA), aliquoted to give 2 × 10 cells per tube, centrifuged at 1000 rpm for 3 min and aspirated.

The hindlimbs were incubated in 12.5% sodium hypochlorite for 10 s and were washed twice with Hank's buffered saline solution and once with L15 medium (Invitrogen Gibco).