The pulmonary artery and left atrium were catheterized and perfused continuously with a solution of 3% bovine serum albumin (BSA) in buffered physiological salt solution (135.5 mM Na+, 119.1 Cl−, 25 mM HCO3−, 4.1 mM K+, 2.8 mM Mg+, 2.5 mM Ca+2, 0.8 mM SO4−2, 8.3 mM glucose).
The neonatal rat cardiomyocytes were placed in buffered physiological saline solution (PSS) containing 140 mM NaCl, 5.9 mM KCl, 1.2 mM CaCl2, 1.4 mM MgCl2, 11.5 mM glucose, 1.8 mM Na2HPO4, and 10 mM Hepes-Tris, to which was added 5 μM fura-2-AM, and the solution was incubated for 1 h in humidified 5% CO2 and 95% air at 37°C.
A7r5 cells were placed in buffered physiological saline solution (PSS) containing 140 mM NaCl, 5.9 mM KCl, 1.2 mM CaCl2, 1.4 mM MgCl2, 11.5 mM glucose, 1.8 mM Na2HPO4, and 10 mM Hepes-Tris, next, 5 μM fura-2 was added to this solution, and then, the cells were incubated for 1 h in humidified atmosphere containing 5% CO2 and 95% air at 37°C.
Groups of 10 animals were collected in plastic multiwells, allowed to equilibrate at room temperature in 300 µl of physiological solution (SolHy) buffered with 1 mM Tris HCl (pH 7, or pH 4, as indicated).
Each thoracic aorta was cooled (4 5 °C) for maximum 8 h in isotonic physiological solution (phosphate buffered saline (PBS, pH 7.4).
Because LB shows auto fluorescence, the cells were harvested and washed and solved in TE-buffer (10 mM Tris-HCl, pH 7.5, 1 mM EDTA), physiological solution (0.9% NaCl) or PBS-buffer.
All experiments were performed using physiological buffered solution at 22°C.
The starting dissolution medium was based on a physiological solution with pH modified by hydrochloric acid or bicarbonate buffer; pepsin was added only to the gastric compartment.
It is a physiological solution as much as it is anything else.
And the films have good corrosion resistance in physiological solution.
