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Other environmental samples were taken with sterile cloths (Sterile cloth, SodiBox), factory pre-impregnated with buffered peptone solution with 10% neutralising agent (lecithin, Tween 80, L-histidine, and sodium thiosulfate).
Sterile cloths pre-impregnated with buffered peptone solution with 10% neutralising agent (lecithin, Tween 80, L-histidine, and sodium thiosulphate), and gloves pre-packed in stomacher bags (SodiBox©, Névez, France) were used [ 38].
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While the detection limit for un-enriched inoculated food samples was 104 CFU/g, this was improved to 10 CFU/g after a 12-h enrichment in buffered peptone water, with 100% reproducibility.
Another suspension was made in buffered peptone water supplemented with cefotaxime (CTX) at 2 µg/mL and then streaked in a Tergitol BCIG plate supplemented with CTX at 2 µg/mL.
Faecal samples were diluted 1 10 with buffered peptone water (BPW) and pre-enriched for 18-24 hours at 41.5°C.
A sample of 400 mg from the ethanol-treated digest content was washed twice with sterile buffered peptone water.
Briefly, samples were initially washed with distilled water and with 90%% alcohol, and after chopping, 10 g of each sample were blended along with 90 ml buffered peptone water (BPW).
Samples (1 mL) were withdrawn every 20 minutes for 1 h and diluted with 0.1% buffered peptone water (BPW, Difco).
To detect campylobacters, portions (10 g) of each faecal sample were diluted with 90 ml buffered peptone water (BPW; Oxoid) and mixed for 1 min.
Salad in each sterile bag was then mixed thoroughly with 225 ml of buffered peptone water.
The samples were put in a sterile universal bottle filled with 225 ml of buffered peptone water.
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