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Rumen fluid was added to the buffered mineral solution with constant stirring, while maintained in a water bath at 39 °C.
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Buffered mineral solution (Menke and Steingass 1988) was prepared and placed in a water bath at 39 °C under continuous flushing with CO2.
Buffered mineral medium with 40 g glucose/l and yeast extract/peptone/dextrose (YPD) complex medium with 17 g glucose/l were used.
Buffered mineral medium contained (per litre): (NH4 2SO4, 15 g; KH2PO4, 28.8 g; MgSO4·7H2O, 1 g; Antifoam 289, 0.2 ml; trace metal solution, 4 ml; vitamin solution, 2 ml and 40 g glucose.
Buffer and mineral solution [ 26] was prepared and placed in a 39°C water bath under continuous flushing with CO2.
Sections incubated with 1% non-immune serum phosphate buffered solution (PBS) solution served as negative controls.
Hind feet were dissected and immersed in transfer buffer (Hank's buffered salt solution supplemented with 10 mM HEPES, pH 7.4).
Plants were watered and fertilized daily with MS mineral solution.
Afterwards, the mineral solution was prepared using the corn cob extract enriched with 1% glucose as carbon source.
trace mineral solution.
Droplet manipulation is also demonstrated with paramagnetic beads and buffer solutions with proteins.
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