Slides were incubated with 0.1% Triton X-100 buffered in phosphate-buffered saline (PBS) for 3 min, and washed three times with PBS at room temperature.
Surgical specimens were paraffin embedded after 4% formalin fixation (buffered in phosphate-buffered saline), and 1- μm slices were prepared for hematoxylin-eosin (HE) staining.
Analysis of Ca2+ transients under differing free Ca2+ concentrations and exogenous Ca2+ buffering in snail neurones indeed showed that Ca2+ is quite well buffered in snail neurones [24].
It is likely that its effect is being buffered in some way, perhaps with increased atmospheric heat being absorbed by the deep oceans.
The requests are buffered in a FIFO.
Packets from each traffic type are buffered in separate queues.
Write operations are buffered in t.ws as before.
The packets will be buffered in LMAG2 until the mobile node is attached.
Intermediate results are buffered in memory mapper worker and periodically written to distributed file system.
First, all data in S 1 is located and buffered in a temporary migrateList (Line 1).
