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Phenothiazine-pyridyl chalcone, a new internal charge transfer (ICT) probe, has been designed that allows naked eye detection and a reversible, ratiometric 'on off' sensing capability for biologically and environmentally significant Cu2+ in CH3OH H2O (4:1 v/v) under buffered conditions.
On the other hand, more buffered conditions and higher pH prevailing in mesotrophic peatlands have previously been shown to be beneficial to N2 fixation (van den Elzen et al. 2017).
In order to analyse kinase activity of recombinant AmVKR, we developed an in vitro kinase assay in which we measured the ability of immunoprecipitated VKR to autophosphorylate in kinase buffered conditions.
Each coverslip was analysed for a total time not exceeding 1 h to ensure properly buffered conditions (33).
Swelling was induced by the addition of calcium gluconate to give the free Ca2+ concentration required under buffered conditions.
21 To prevent any oxidation of the arylamine by HRP at low pH, 22 all assays were carried out in pH 7.4 buffered conditions.
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We used lipid and buffer conditions known to procure the membrane insertion and channel formation as reported previously [17].
Protease sample concentrations, buffer conditions, data acquisition, and data processing were as previously described.
Antibodies may require very different protocol and buffer conditions in order to reach their optimal performance.
Identifying optimal detergent(s) and buffer conditions for protein stability is, however, often difficult and time consuming [4,5].
As a result, the same oligonucleotide constructs could be used multiple times with different polymerases and buffer conditions.
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