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M199 medium (Gibco BRL) was buffered at the indicated pH using 150mM HEPES.
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Proteins were added to this buffer at the indicated concentrations.
The kinetic constants in the pH range 7 11 were measured as described above, with the exception that the reactions were carried out in sodium phosphate buffer at the indicated pH values (5.0, 6.0, 7.0, 8.0, 9.0 and 10.0).
For pH optimum determination, substrate was dissolved in 50 mM Tris-acetate buffer containing 150 mM NaCl at the indicated pH values.
Confluent MDCK cells were infected with virus at moi 2 in MES buffer (0.25 µg/ml Amph B) at the indicated pH values or mock-infected.
Reactions were terminated by adding SDS sample buffer at the time indicated.
Red blood cells were lysed in NH4Cl buffer; subsequently, the cell pellets were recovered and infected with OBP-401 at the indicated concentrations.
cAMP was added at the indicated concentrations.
Tensile testing of SPU films at 37 °C in phosphate buffered saline (PBS) indicated that the Young's modulus and elongation of the polyurethane copolymers could be tailored by altering the soft segment block length.
Although the concentration of purgeable Hg produced in samples buffered at pH 6 and 8 was monitored only under dark conditions, the concentration of purgeable Hg did not differ significantly (p = 0.60 using a two-tailed Student's t test) under illuminated versus dark conditions in samples buffered at pH 7 indicating that Hg II) reduction occurred to a similar extent regardless of illumination.
Appropriate stock buffer pH was empirically determined, as necessary to adjust the pH of YPD liquid media at room temperature to the indicated final pH.
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