Sentence examples for buffer with the supplement from inspiring English sources

Supernatants were collected and pre-cleared with Dynabeads G in ChIP lysis buffer with the supplement of 100 μg BSA and 100 μg ssDNA.

After that, the buffer was replaced to TE buffer with no supplement and stored at 4 °C.

Beads were washed four times in ELB buffer (with supplements) and stored dry at −80°C until IB analysis.

A 1 10 dilution was produced by adding 1 ml of Butterfields phosphate buffer to 9 ml of Butterfields buffer supplemented with the neutralizing agents.

Two attempts were made to purify nNOS with pterin-free buffer or the buffer supplemented with 1, but the protein denatured completely upon trypsinolysis required for generating the heme domain.

All titrations were carried out at 22°C in either G-buffer or G-buffer supplemented with the KCl concentrations indicated and a five-fold molar excess of LatA (Biomol) to actin.

Whole-cell lysates were prepared by washing the cells with phosphate buffered saline (PBS) and subjecting them to lysis with Laemmli sample buffer supplemented with the protease inhibitor cocktail.

MBP-tagged protein was eluted with the buffer given above supplemented with 10 mM maltose.

Proteins are extracted from the still frozen pulverised tissue by addition of Tris-buffered saline to obtain the cytosol fraction of the tumour or by the Tris buffer supplemented with the non-ionic detergent Triton X-100, and, after high-speed centrifugation, are found in the tissue supernatant.

Cells were then washed 3 times in 1x PBS pH 8.0 and lysed in RIPA buffer supplemented with the Complete Protease Inhibitor Cocktail, EDTA-free (Roche).

A 1 10 dilution of each hand rub was produced by adding 1 gram of a hand rub to 9 ml of Butterfields buffer supplemented with the neutralizing agents.