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The sample was then washed with a buffer with the same content except for 10 mM NaNO3 and was concentrated to ∼2.5 mg of Chl/mL.
The assays were performed according to the manufacturer's instructions, and Aβ standards were prepared in a buffer with the same composition as the samples.
As an example, we studied the acidification behavior of a 25 mM HEPES buffer with the same components and starting pH as the 25 mM Tris buffer used in Figure 3A.
It should also be noted that the BglMKg enzyme lost enzymatic activity in a 100 mM Tris buffer (Table 6), while changing to a 100 mM sodium phosphate buffer with the same pH restored the activity to 90% of that in the buffer C (a phosphate buffer used in purification procedure, Methods).
Cells were washed three times with ice-cold Kreb's buffer with the same concentrations of glucose as treatments and lysed in ATP-releasing buffer (Sigma), followed by centrifugation at 12,000 × g for 5 min. The resulting supernatants were used immediately for measurement of ATP using an ATP Bioluminescent Assay Kit (Sigma).
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In Figure 11, all nodes are given buffers with the same capacity which is varied from 0 to 2 kbit.
The pellet (nuclei) was washed with cytosolic buffer, resuspended with the same buffer volume as the sup and subjected to western immunoblot.
We examined the different optimal thresholds for different buffer sizes with the same offered load.
In fact, using a 170 mK 3He buffer gas with the same density, we still estimate the steady-state ion temperature to be ∼10 K.
Different pH regulators divided into acidifying agent, alkaline and buffer salt with the same addition was selected and applied to the RSHH medium, respectively.
Control mice were given a placebo of injection buffer only with the same frequency.
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