Sentence examples for buffer with the proteinase from inspiring English sources

The cells were collected in ice-cold PBS, and the cell extracts were prepared in RIPA buffer with the proteinase inhibitor cocktail from Sigma (St . Louis MO).

Then, 100 mg of intestinal tissue was weighed into a 1.5-ml microcentrifuge tube containing 0.5 ml of TE buffer with the proteinase inhibitor Cocktail (Roche, USA).

Macrodissected FFPE and fresh frozen tissues were digested over-night at 56 °C in ATL buffer with the addition of proteinase K (Qiagen).

Notably, supplementation of the extraction buffer with a proteinase inhibitor cocktail did not prevent proteolysis (Fig. 1B).

Protein was extracted from tissue samples of the patient and the controls after homogenization in RIPA buffer with a proteinase inhibitor cocktail (Complete®, Roche-Diagnostics, Basel, Switzerland), 50 µg protein were separated through denaturating SDS-PAGE with the Laemmli system and blotted on nitrocellulose membranes by the semidry method (Biometra, Göttingen, Germany).

Cells were grown in complete media overnight, treated with dasatinib for 2 48 h, washed, collected by scraping, and lysed in 1% NP-40 buffer supplemented with the appropriate proteinase and phosphatase inhibitors.

Cell were precipitated by spinning at 3000 rpm at 4°C for 5 min and incubated with the lysis buffer with proteinase inhibitor on ice for 15 min. Tubes were spun, the nuclear lysis buffer with proteinase inhibitor was added and sonicated to form shared chromatin of about 200 1000 base pairs in length (data not shown).

The pellets were resuspended in lysis buffer with added proteinase K (Sigma-Aldrich, USA) and ≤106-μm-diameter glass beads (Sigma-Aldrich, USA).

Whole individuals were first soaked overnight in the extraction buffer with proteinase K at 56°C, leaving the gut and chitinous body parts intact.

Cells were washed and extracted in RIPA buffer with proteinase inhibitors at the indicated time points.

Tissues were lysed in sodium dodecyl sulphate-lysis buffer with proteinase K, and DNA was extracted with the standard phenol chloroform ethanol precipitation method.