Sentence examples for buffer with the peptide from inspiring English sources

For LTx analysis, 50 μL of sample was diluted 1 10 in 450 μL phosphate-buffered saline with 0.05 % Tween20 (PBST), mixed with 6.25 μL anti-PA-MBs for 1 h, washed two times 2 min each in 1.0 mL PBST, then in 200 and 100 μL dH2O for 1 min each, resuspended in 30 μL reaction buffer with the peptide substrate and reacted for 2 and 18 h at 37 °C.

We observed R9-TAMRA labelling of cell nuclei only when we replaced 37°C buffer with 15°C buffer already containing the peptide, i.e. R9-TAMRA was present during rapid temperature change.

The buffer flow was kept constant for 30 min to ensure that the baseline was stable and then the system was stopped for maximum of 1 min and the buffer container was exchanged with the peptide solution containers, after which the solutions were pumped into the system.

They were then imaged in HBSS buffer supplemented with the corresponding peptide at RT. Epifluorescence imaging was performed on a Zeiss Axiovert 200 M Microscope equipped with a xenon lamp and a cooled CCD, under a 40X oil immersion objective.

To start incubation with the peptide, 37°C buffer was rapidly replaced with buffer at the desired temperature (4°C, 15°C, 25°C or 37°C) containing 2 μM R9-TAMRA together with the drugs being tested.

Fluorescamine was added and the amount of peptide in the buffer solution was determined using an external calibration with the peptide fragment.

For each sample, 0.5 µg of digested peptides are loaded onto the trap column at 1 µl/min and desalted with 10 µl of Buffer A. The peptides are separated through a 100 min linear gradient from 100% Buffer A to 30% Buffer B and then going up to 100% Buffer B in 1 min, followed by a 3-min 100% Buffer B wash before returning to 100% Buffer A in 2 min and maintaining at 100% Buffer A for 4 min.

Before reaction termination with SDS sample buffer, the peptides were cleaved from the GST tag with thrombin protease (Amersham) at 25°C for 2 hr.

After overnight digestion, digestion buffer containing the peptides was recovered.

After overnight digestion, buffer containing the peptides was recovered.

In diH2O the peptides interacted strongly with HA and chondroitin sulfate, but in phosphate buffered saline the peptides interacted more strongly with HA.