Sentence examples for buffer with the pH from inspiring English sources

Figure 5c, d shows the optimum buffer capacity, and ionic strength were achieved using 0.05 M of sodium phosphate buffer with the pH fixed at pH 7.0 and 2.0 M of NaCl, respectively.

For measuring pH optimum and pH range, a 50 μl aliquot of enzyme solution was incubated with 50 μl of 100 mM buffer with the pH set to different pH values between 2.5 and 10.0 in steps of 0.5: citrate (2.5 5.5), carbonate (5.3 7.3), phosphate (6.2 8.2), Tris (7.5 9.0) and glycin (8.8 10.0).

Tests for the determination of temperature optimum were performed with 5 mM pNP-β-GlcNAc/pNP-β-GalNAc at different temperatures in a Gradient PCR Thermocycler (Eppendorf, Germany) or thermomixers in McIvaine buffer with the pH as determined to be optimal for the specific hexosaminidase.

The hydrolysis was carried out in closed 15-mL Falcon tubes in triplicates in 5 mL total volume containing 2% substrate (20 mg dry substrate per mL) suspended in 50 mM sodium citrate buffer with the pH adjusted to 5.0, in a 45°C water bath shaken at 100 rpm.

NAD+ was dissolved in a buffer with the desired pH, and phosphite stock solutions were adjusted to the pH of the assay.

The proposed circuitry applied to Si3N4 and Al2O3-gate ISFETs demonstrate a variation of the drain current less than 0.1 μA and drain–source voltage less than 1 mV for the buffer solutions with the pH value changed from 2 to 12.

Two buffer systems with the pH range of 3.5 9.0 (15 mM acetic acid, 15 mM Mes and 30 mM Tris) and 5.7 10 (30 mM Aces, 15.6 mM Tris and 15.6 mM ethanolamine) were used.

It should also be noted that the BglMKg enzyme lost enzymatic activity in a 100 mM Tris buffer (Table  6), while changing to a 100 mM sodium phosphate buffer with the same pH restored the activity to 90% of that in the buffer C (a phosphate buffer used in purification procedure, Methods).

Experiments were carried out in a closed double-deck flask, containing in the first stage 50 mL of 2 M NaHCO3/Na2CO3 buffer with the appropriate pH to obtain the desired CO2 concentration [ 38, 39], and containing in the second stage 70 mL of culture medium.

Cultivation was performed in a closed double-deck flask, which had a first stage containing 50 mL of 2 M NaHCO3/Na2CO3 buffer with the appropriate pH to obtain the desired CO2 concentration of 1% (v/v), and a second stage containing 70 mL BG11 culture medium [ 30, 31].

Alternatively, PCA and PCD were added to a buffer with the same starting pH, containing 10 μM SNARF-1, 5 mM MgCl2, 2 mM Trolox, and 25 mM Tris.