Sentence examples for buffer with the indicated from inspiring English sources

For immunostaining experiments, fixed cells were permeabilized in PBS containing 0.1%NP40and5% 5% normal donkey serum and then stained in the same buffer with the indicated antibodies.

Pools of LB226692 OMV OptiPrep fractions 1 to 6 and 4 to 7, respectively, were incubated in HEPES buffer alone (control), or in HEPES buffer with the indicated chemicals.

The beads were then boiled in 1% SDS loading buffer for western blotting with the indicated antibodies.

Proteins were added to this buffer at the indicated concentrations.

Immunoprecipitated protein complexes were washed four times in lysis buffer, eluted by heating in LB sample buffer, resolved on SDS-PAGE and immunoblotted with the indicated antibodies.

The bound proteins were eluted in 2x Laemmli buffer, resolved by SDS-PAGE, and western blotted with the indicated antibodies.

Complexes were washed with lysis buffer and subjected to SDS-PAGE and immunoblot with the indicated antibodies.

After 3 washes with ice-cold lysis buffer, proteins were eluted in reducing Laemmli's buffer, resolved by SDS-PAGE, transferred to nitrocellulose and immunoblotted with the indicated antibodies.

Thirty micrograms of total protein were mixed with SDS sample buffer, separated by SDS PAGE and subjected to western blot analysis with the indicated antibodies.

After being clarified with centrifugation, lysates were boiled in SDS sample buffer, separated by SDS PAGE, transferred to polyvinylidene difluoride membranes and probed with the indicated antibodies.

To examine p38 and ERK1/2 phosphorylation, untreated and treated cells were extracted with the buffer indicated above.