Figure 3c show three different calibrations, all carried out with the identical sensor and buffers.
Since the M Fano decoders and the N input buffers are identical to each other, the system is totally symmetric and can be treated as a faster Fano decoder with the clock speed of f c l k ′ = M ⋅ f c l k working on each input buffer with the probability of P S = 1/N.
We also performed fluorescence anisotropy titrations in Buffer C, which is the identical high-salt buffer used by Brennan and coworkers i.e.e
In all experiments concentrated stocks of peptides (IC3-C58, IC3-M28, IC3-C30, and IC3-C30) were made up in the appropriate buffer (with and without calcium), identical to that of the S100B protein.
After loading, the tissues were placed in a small chamber between two platinum wire electrodes and superfused for 1 h with superfusion buffer (which was identical to the low-calcium buffer, except that the [CaCl2] was increased to 2.5 mM).
Rule 4 is 'regard the buffer colors as identical to the neighboring color corresponding to faster healing time'.
In our previous study, we determined Kd,n to be 16 μM for Egr-1 under the identical buffer conditions with 150 mM KCl.
As a negative control the motor domain and the CTE712C domain were incubated in the identical buffer, supplemented with 5 mM DTT. Tubulin (5 mg/ml) was first polymerized into MTs in the presence of 1 mM GTP and gradual addition of 0.05 µM, 0.5 µM and 2 µM taxol for 1 hr at 37°C.
Buffer injections identical to the analyte were included throughout the analysis for the purpose of double referencing.
6-Hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) standard (0.25 50 µM) was diluted in the identical phosphate buffer.
