Sentence examples for buffer with the appropriate from inspiring English sources

For further separation of CD4+ CCR6+ lymphocytes 5×107 cells per sample were incubated in 500 µl of staining buffer with the appropriate antibodies.

After fixation/permeabilization cells were washed with 0.5 ml of 1x BD Perm/Wash buffer (BD Biosciences; alternatively with PBS-BSA) and resuspended in 50 μl of 1x BD Perm/Wash buffer with the appropriate dilution of primary antibodies.

Cell pellets were then resuspended in 50 μl of 1x BD Perm/Wash buffer with the appropriate dilutions of secondary antibodies, and incubated for 30 min at room temperature in the dark.

Experiments were carried out in a closed double-deck flask, containing in the first stage 50 mL of 2 M NaHCO3/Na2CO3 buffer with the appropriate pH to obtain the desired CO2 concentration [ 38, 39], and containing in the second stage 70 mL of culture medium.

Cultivation was performed in a closed double-deck flask, which had a first stage containing 50 mL of 2 M NaHCO3/Na2CO3 buffer with the appropriate pH to obtain the desired CO2 concentration of 1% (v/v), and a second stage containing 70 mL BG11 culture medium [ 30, 31].

Cells were grown in complete media overnight, treated with dasatinib for 2 48 h, washed, collected by scraping, and lysed in 1% NP-40 buffer supplemented with the appropriate proteinase and phosphatase inhibitors.

The RNA gels were transferred to BrightStar-Plus nylon membranes (Ambion), crosslinked using a UV crosslinker, and prehybridized, then hybridized overnight in the ULTRAhyb-Oligo Hybridization Buffer (Ambion) with the appropriate DIG-labeled probe at 42°C.

Membranes were washed 4 times, 20 min each with antibody incubation buffer then incubated with the appropriate secondary antibodies (BioRad) diluted 1∶10000 in Buffer A for 2 hr at RT then washed 4×20 min each with Buffer A and once for 5 min with PBS.

The membranes were washed extensively in the washing buffer and incubated with the appropriate horseradish peroxidase-conjugated secondary antibodies (Sigma-Aldrich, Singapore).

The blots were washed thrice in wash buffer and incubated with the appropriate horseradish peroxidase-linked secondary antibodies.

The membranes were washed in PBS Tween-20 buffer and incubated with the aPBS Tween-20RP-conjugated secondary antibuffer