Sentence examples for buffer with the addition from inspiring English sources

HPV was well elicitable and most constant using Krebs–Henseleit buffer with the addition of hydroxyethylamylopectin as an oncotic agent.

In brief, 5 μg of total urine proteins for each group were mixed with the Laemmli sample buffer with the addition of β-mercaptoethanol as reducing agent.

Both MBP/exochitinase (MBP/exo) and MBP/endochitinase (MBP/endo) linked to the maltose were then eluted using column buffer with the addition of 10 mM maltose.

Thus, we choose TB medium (12 g/L tryptone, 24 g/L yeast extract, 4 g/L glycerol, and 100 mL/L PBS buffer) with the addition of 10 g/L MOPS as the initial SrtA fermentation medium.

To investigate the storage stability, a special buffer with the addition of stabilizers and preservatives (10 mM phosphate buffer, pH 7.4 + 1 mM EDTA + 1 mM dithiothreitol + 0.1%% NaN3) was used.

Artificial saliva was prepared using saliva buffer with the addition of 0.3 mg/mL human recombinant lysozyme (Sigma, L1667) and 0.021 mg/mL mucin from bovine submaxillary glands (Sigma, M3895).

Cytoskeleton was prepared in microtubule stabilizing buffer (MSB) with the addition of protease inhibitors (Complete Mini, Roche).

Next, the nuclease-containing material was subjected to size-exclusion chromatography employing Sephacryl S-200 HR and the previously defined buffer A with the addition of 0.15 M NaCl.

The suspension was centrifuged, and the pellet resuspended in 20 µl of buffer A with the addition of Nonidet-P40 (to 0.15% v/v) and incubated on ice for 10 mins.

Following the general procedure of Wan and Wilkins, RNA was isolated using the XT buffer system with the addition of chloroform/iso-amyl alcohol extractions and LiCl precipitation steps [ 63].

Total RNA was isolated using the XT buffer system with the addition of chloroform/iso-amyl alcohol extraction and LiCl precipitation steps [ 85]. Isolation of total RNA and mRNA purification is described previously [ 39].