Sentence examples for buffer with cocktail of from inspiring English sources

Cells or tissues were washed with phosphate buffered saline (PBS) and lysed in RIPA buffer with cocktail of protease inhibitors (Roche).

Total proteins were extracted from the half of brain by homogenization in RIPA buffer with cocktail of protease inhibitors (Roche).

Whole cell extracts were prepared in RIPA buffer with cocktail of protease inhibitors and reducing agents (NaVO3 1mM, DTT 1mM, PMSF 1mM, proteinase cocktail inhibitor ROCHE Cat. No. 11836153001).

Cells were lysed in RIPA buffer complemented with cocktails of protease and phosphatase inhibitors (Sigma-Aldrich).

Subsequently, cells were washed with FACS buffer (PBS, 0.5% BSA, 0.05% NaN3), pelleted, and stained with cocktails of fluorochrome –conjugated antibodies (Table S9).

Cells were harvested after 24 or 48 h, then lysed in CelLytic M lysis buffer (Sigma), supplemented with cocktail tablets of protease (Roche - Complete Mini EDTA-free) and phosphatase inhibitors (Roche – PhosSTOP).

Whole-cell extracts were made with RIPA buffer supplemented with cocktail inhibitors as above described.

Resting platelets were lysed with 1% Triton X-100 containing lysis buffer supplemented with a cocktail of protease and phosphatase inhibitors.

RAW 264.7 cells or BMDCs were harvested, pelleted, washed with PBS and lysed in RIPA (Radioimmunoprecipitaiton Assay) buffer (Sigma Aldrich) with a cocktail of proteinase and phosphatase inhibitor (Thermo Scientific, Waltham, MA).

The powder was extracted at 4°C with 0.1 M phosphate buffer containing a cocktail of protease inhibitors (Roche).

Briefly, B/TSM cells were lysed for 30 min 4°C in NP-40 lysis buffer supplemented with a cocktail of protease inhibitors (2 mM sodium orthovanadate, 1 mM phenyl-methylsulfonylfluoride, 10 µg/ml leupeptin, 0.15 units/ml approtinin, 1 µg/ml pepstatin A) (Sigma-Aldrich) and centrifuged for 20 min to remove nuclei.