Sentence examples for buffer with addition of from inspiring English sources

In the refolding experiments, the reduced and denatured protein was rapidly diluted into the refolding buffer, with addition of different additives at an appropriate concentration, and kept in sealed tubes at various temperatures.

After four additional washes with 200 μl of this buffer with addition of 5 mM Mg-ATP and one further wash with 100 μl of 20 mM Tris HCl pH 7.5, the proteins were eluted by a pH shift; 100 μl 0.1 M triethylamine pH 11 were added to the columns, and eluates were collected in tubes containing 13 μl 1 M MES pH 3 for neutralisation.

However, when the same protein was run in the same buffer with addition of 150 mM NaCl, we found that the peak was shifted to a range at which high molecular weight proteins were eluted (Figure 3D).

The protein was eluted with an elution buffer, which was the lysis buffer with addition of 250 mM imidazole (pH 8.0).

The pellet was solubilized in the same buffer with addition of 1% SDS for 10 min at RT and, after centrifugation at 12,000  g for 15 min, the supernatant was collected as detergent-insoluble fraction.

After extensive wash, the beads were resuspended in 40 μl kinase buffer with addition of cold ATP (30 μM), 8 ng of substrate (GST-c-Jun), and 10 μCi of (γ-32P ATP.

Synaptosomes were washed gently twice and solubilized for 1 hr at 4°C in IP buffer containing HKA buffer with the addition of 2% freshly prepared y3-[ 3-cholamidopropyl dimethylammonio]-1-propanesulfonic acid (CHAPS).

Incubated cells were washed 3 times with PBS and lysed with standard cell extraction buffer (Invitrogen) with addition of a standard mixture of proteinase inhibitors (Sigma).

Lung tissue and PASMC's samples were homogenized using tissue homogenizer (for tissues only) or lysed in RIPA buffer (Santa Cruz) with addition of a protease inhibitor cocktail and PMSF.

Cells and frozen tissues were homogenised in NP-40 Lysis buffer (Life Technologies) with addition of protease inhibitor (Complete protease inhibitor cocktail; Roche) and 1 m M of phenylmethanesulfonyl fluoride (Sigma-Aldrich, St Louis, MO, USA).

EPR spectra were obtained at ∼100 μM labeled protein ± 2 mM AMPPNP in the buffer above with addition of 2 mM MgCl2 and after heating at 30°C for 30 min to ensure closure has completed.