Sentence examples for buffer with a thiol from inspiring English sources

The sample was then incubated on the gold surface for 24 h under the following conditions: 25 °C TE buffer with a thiol DNA concentration of 2 μM.

The plasma thiol compounds were reduced with tri-n-butylphosphine and derivatized with a thiol specific fluorogenic reagent: ammonium 7-fluoro-benzo-2-oxa-1,3-diazole-4-sulphonate.

These reagents react rapidly with a thiol through a thiol-specific thiol sulfide exchange reaction (Scheme 1).

These nonfluorescent benzofurazan sulfides react rapidly and specifically with a thiol to form a strong fluorescent thiol adduct.

As a fluorogenic agent, GUALY's reagent itself exhibits very minimal fluorescence but forms a thiol adduct with strong fluorescence after reacting with a thiol.

Reaction of this electrophile with a thiol adds the following moiety CH3SO2NHN=CHCH2– to the sulfur atom.

A few high-throughput assays for GSH, homocysteine and cysteine, cysteinylglycine, and total thiols with a thiol-selective agent, 5- bromomethyl)fluorescein, have been reported.

Valproic acid was functionalized with a thiol-reactive group, which was further reacted with the dendrimer.

This can be achieved using high irradiation intensities and special chemical buffers with thiol compounds and oxygen removal.

This effect was abolished by preincubation of isolated mitochondria with DTT, a thiol reducing agent.

DTT was then removed by three cycles of dilution with 0.2 mL buffer A and ultrafiltration, and the N-Hcy-protein preparation was diluted to 50 mg protein/mL with buffer A. The thiol content in N-Hcy-protein was quantified according to Ellman (1959).