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All probe molecules not incorporated into liposomes formed aggregates in the buffer with a specific spectral response.
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Membrane fragments were washed four times with the same buffer and solubilized with a specific phosphate buffer (see below).
The pellet was washed with distilled water, pelleted again and resuspended in distilled water before mixing with 10 ml citrate buffer, pH 2.5, supplemented with a specific amino acid.
The RNA was blotted on a membrane (Hybond-N, Amersham Biosciences) and hybridized (UltraHyb hybridization buffer, Ambion) with a gene-specific probes (213, 245 and 314 bp) designed from the 3' ends of GRCD3, GRCD4 and GRCD5 cDNA molecules, respectively.
In the present study, we analyzed associations of NO2, PM2.5, and two GIS (geographic information system) indicators of traffic exposure (distance to heavy traffic roads with > 10,000 vehicles per day, and traffic intensity in a 150 m buffer) with cause-specific mortality in adults included in the Rome Longitudinal Study (RoLS; Cesaroni et al. 2010).
Slides were then re-dehydrated and prehybridized in hybridization buffer with 0.5 nm specific probe (LNA-modified and digoxygenin (DIG -labeled oligonucleotiDIG -labeledColigonucleotideark) complExiqonry to miR-124.
It was with a specific purpose.
Train with a specific trick in mind.
The buffer has a periodic timer, and each packet is stored in the buffer for at least a specific period.
The buffers were titrated to a specific pH with KOH solution, and the overall ionic strengths were fixed with KCl.
In high latitude countries the buffer space should be of a specific design containing two cuboid sub-spaces with specific internal overhang and a well-planned extension of the south glazed facade.
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