Sentence examples for buffer with a protease from inspiring English sources

For substrate-trapping experiments, lysates were prepared in 1% NP40 buffer with a protease inhibitor cocktail (without sodium orthovanadate) and hPTP1B was immunoprecipitated using FG6 antibodies.

They were then washed with PBS containing 50 µM sodium orthovanadate and resuspended in lysis buffer with a protease inhibitor cocktail (Roche Diagnostics).

An aliquot of capacitated spermatozoa was centrifuged at 1,500× g for 5 min and the pellet was resuspended at 6×106 cells per 10 µl of radio immunoprecipitation assay (RIPA) buffer with a protease inhibitors cocktail.

Cells were harvested by centrifugation at 4°C, washed in 50 ml CBB buffer (20 mM Tris-Cl pH 8.0, 25 mM NaCl, 5 mM EDTA, 3.6 mM β-mercaptoethanol), and resuspended in 10 ml CBB buffer with a protease inhibitor cocktail (Complete EDTA free, Roche).

Later, cells were collected in a non-reducing sample buffer with a protease inhibitor cocktail.

The spinal cord was lysed in ice-cold RIPA buffer with a protease inhibitor cocktail (Sigma Aldrich, St . Louis MO).

To prepare whole cell lysates, cell pellets were washed in PBS and lysed using standard RIPA buffer supplemented with a protease inhibitor cocktail (1∶50 dilution of lysis buffer).

Cells were stimulated with anti-CD3ε (clone HIT3a; 1 µg/mL, BD Biosciences, San Diego, CA)+anti-IgG2a (1 µg/mL, Southern Biotechnology Assoc., Birmingham, AL), as indicated, and lysed in RIPA buffer supplemented with a protease inhibitor cocktail (Boehringer, Ridgefield, CT), 10 mM NaF, and 1 mM Na3VO4.

Western blot was performed from lysates obtained using RIPA buffer supplemented with a protease inhibitor cocktail (Sigma).

Total cell lysates were prepared in RIPA lysis buffer supplemented with a protease inhibitor cocktail (Roche, Bazel, Switzerland).

Protein extracts from LV tissues were prepared by adding ice-cold RIPA buffer supplemented with a protease inhibitor cocktail (Roche, Basle, Switzerland), and homogenised using a TissueLyser II (Qiagen).