Sentence examples for buffer with a homogenizer from inspiring English sources

Tumor tissues were resected and lysed in lysis buffer with a homogenizer (HM-100; Sysmex Co .. Welch's t test was used for comparison.

The chloroform was evaporated under a stream of nitrogen, the lipids were resuspended in PBS buffer with a homogenizer to a final concentration of 20 mg ml− 1 and frozen in liquid nitrogen.

Brains and livers dissected from E14.5 mouse embryos were homogenized in RIPA buffer with a Dounce homogenizer, followed by centrifugation at 13,000 g at 4°C for 15 min. MEFs and ESCs were also lysed in RIPA buffer followed by centrifugation (13000 g, 151 min) after 3 freeze-thaw cycles.

Briefly, ∼50 mg samples were weighed and homogenized in lysis buffer with a Dounce homogenizer with 2 mM phenylmethylsulfonyl fluoride (PMSF) added.

Each section of intestine was homogenized in 10 mL of 100 mM potassium phosphate buffer, pH 6.8, with a homogenizer (Ultra-Turrax T25, Janke & Kunkel Co., Staufen, Germany).

The kidneys of 12 16 wk old db/+ and db/db mice were collected, and cleaned slices (10 25 mg) of kidney tissues were homogenized in 1 ml RIPA buffer with a glass-glass homogenizer manually for 30 strokes.

All brain tissue samples (n=6 per experiment for H–I and sham) were dissected fresh and pooled [IL (ipsilateral) and CL (contralateral) were pooled separately for H–I brains] and homogenized on ice in 500 μl of homogenization buffer with a Tissue-Tearor (rotor/stator type) homogenizer (BioSpec Products).

These tissues were homogenized in a buffer solution (100 mg of tissue per mL of RIPA lysis buffer), with the homogenizer immersed in an ice bath.

Cells were centrifuged (100× g, 3 min, 4 °C) and homogenized with 3 volumes of freshly made buffer A with a Dounce homogenizer.

Cells were centrifuged (1,000× g, 3 min, 4 °C) and homogenized with 3 volumes of freshly made buffer A with a Dounce homogenizer.

The brain of rats were dissected and homogenized with a homogenizer in ice-cold Phosphate buffer (50 mM, pH 7.4) to produce a 1/10 homogenate.