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These compounds degrade rapidly in pH 8 buffer with a half life of 1 2 h.
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The prodrug is stable, being slowly hydrolyzed in pH 7.6 buffer at 37 °C with a half-life of 37 h.
97% release of diacerein was achieved in phosphate buffer (pH 7.4) with a half-life of 48 min, following first order kinetics.
Crystals were then briefly exposed to crystallisation buffer (with half fragment concentration and an additional 10%% glycerol) as a cryoprotectant and flash cooled at 100 K in liquid nitrogen.
PCR products were mixed with a half volume of loading buffer.
The perfusion was carried out with a half- synthetic medium on the basis of Krebs- Ringer- bicarbonate buffer (KRB), pH 7.38 [ 11].
He straightens it with a half turn.
John Mills, another veteran of starchy, upper-class parts, is a twinkling old buffer with a nose for "naughty salt".
Upon combining furimazine with the assay buffer, the working solution at ambient temperature loses potency with a half-life of ∼2 days.
After the ceasefire, each side walked back two thousand metres, creating the D.M.Z., a buffer zone two and a half miles wide.
Each enzyme sample (3 μL/12 μL) was adjusted to desired pH by adding a half volume of buffer with different pHs: 0.2 M citrate buffer (pH 4.0), 0.2 M phosphate buffer (pH 5.0, 6.0, 7.0, 8.0, or 9.0) or 0.2 M CAPS buffer (pH 10.0).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com