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Mixer performance is characterized by mixing buffer with a fluorescence tracer containing fluorescein.
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After washing thrice with Apoptosis Wash Buffer, the cells were suspended in 300 μl buffers and analyzed with a fluorescence microscope in three independent experiments.
Then, the cells were washed thrice with PBS buffer and directly imaged with a Fluorescence Microscopy (BX51, Olympus).
1. Prepare a 1-21-2%arose gel (e.g., Lonza SeaKem LE) in 1X TAE or TBE buffer with available fluorescence dye (e.g., SYBR Safe).
The absorbance of the samples added to the DMMB buffer was read at 595 nm with a fluorescence reader (Molecular Devices).
Thereafter, nodules were washed three times for 15 min each with buffer A and fluorescence was detected with a Zeiss LSM 510 laser scanning confocal microscope exciting at either 488 or 543 nm.
The blood mixed with buffer was sonicated for 3 min, and its fluorescence measured with a fluorescence microplate reader (Tecan Japan Co., Ltd).
A lysis buffer and dye solution was added and the plate was read with a fluorescence plate reader using a 480/520 nm filter set (Thermo Labsystems, Cheshire, England, UK).
An additional mouse was injected with 200 µl of dye in Tris buffer with fluorescence that was equivalent to that of the high-dye Ad.
On a C18 column, the derivatives of APF with seven free fatty acids could be separated completely in 22 min using a mobile phase of methanol water (88 12, v/v) containing 7 mmol L−1 pH 6.5 Na2HPO4 H3Cit3 buffer with fluorescence detection at λex/λem = 467/512 nm.
As the name suggests, "Baby Kraken" is an image of a squid embryo taken with a fluorescence microscope.
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