Sentence examples for buffer with a flow from inspiring English sources
Samples were eluted with 0.025 N H2SO4 isocratic elution buffer with a flow rate of 0.5 ml/minute.
100 mL of each sample were injected and separated by using PBS as running buffer with a flow rate of 0.5 mL/min.
HBS buffer (10 mM HEPES pH 7.4, 0.15 M NaCl, 3 mM EDTA, 0.005% (v/v) Surfactant P20; Biacore AB) was used as the running buffer with a flow rate of 10 µl/min.
The GPC elution buffer was a phosphate buffer with a flow rate of 0.75 mL/min.
The concentrated protein was then run on a Superdex 75 10/300 GL (GE) gel filtration column in the same buffer, with a flow rate of 0.3 mL/min to remove impurities.
The mobile phase consisted of 100 mM KH2PO4 and 1.0 mM tetrabutylammonium sulfate (TBAS) at pH 6.0 (buffer A) and CH3CN (buffer B) with a flow rate of 1.0 mL/minute over an Eclipse Plus C18 column with 5 μM diameter beads, 4.6 × 150 mM in length (Agilent Technologies).
The mobile phase was a mixture of acetonitrile and Na2HPO4-citric acid buffer 1 (20/80, v/v) with a flow rate of 1 mL/min, in which Na2HPO4-citric acid buffer 1 was composed of 19.4 mmol/L NaH2PO4 and 6.1 mmol/L citric acid.
The crude laccase extract was further purified by three successive steps of ion exchange chromatography: firstly on DEAE-cellulose (10 mM phosphate buffer, pH 7.0) with a flow rate of 2 mL/min, secondly on CM-cellulose (10 mM phosphate buffer, pH 6.6) with a flow rate of 2 mL/min, and finally on Q-Sepharose (10 mM sodium acetate buffer, pH 4.0) with a flow rate of 1 mL/min.
At each different node, the tagged flow is multiplexed into a FIFO buffer with a different interfering flow.
For interaction measurements, 70 µl samples containing different concentrations of the analyte were injected on the sensor-chips at a flow rate of 20 µl/min, followed by wash with buffer at a flow rate of 20 µl/min.
The eluent consisted of 0.1 M of Na2SO4 in 0.1 M of phosphate buffer (pH 6.7) with a flow rate of 1 ml min-1, with detection carried out using an RI detector.
