Sentence examples for buffer with a final from inspiring English sources

To study the separation and detection of the bacteria from the blood cells, a 1× phosphate-buffered saline (PBS) buffer diluted with the 300-mM sucrose solution in a 1 15 ratio was used for the experimental buffer with a final conductivity of 1 mS/cm, owing to the fact that blood cells are highly sensitive to the osmotic pressure of a solution.

Water channel activity of reconstituted mAQP4 M23 samples obtained from either P-CF or D-CF expression mode was analysed in a 100 µl reaction mixture, which was composed of 50 µl proteoliposomes and 50 µl reconstitution buffer with a final sucrose concentration of 200 mM.

The solution was diluted 8-fold into an aqueous buffer with a final concentration of 25 mM Tris-HCl, pH 8.3 and 1 mM CaCl2 and digested with 5.5 µg/mL of sequencing-grade modified trypsin (Promega, Madison, WI) (enzyme/substrate ratio of 1∶250) for 16 h at 37°C.

Aggregation of human platelets under high shear conditions was assessed using a standard optical aggregometer (Lumi- Dual Aggregometer; Chrono-Log, Havertown, PA, USA) at 37°C and 1200 rpm in a volume of 500 mL buffer with a final platelet concentration of 6.5×105 platelets/ml over a 5 min interval.

Sliding between MTs was observed in the same buffer with a final concentration of 100 mM K-acetate.

After centrifugation, the pellet was resuspended in hypotonic buffer with a final concentration of 0.5%NP-40NP-40nidet P-40 (FlukAGAG, Buchs, Switzerland); to lyse nuclei.

Buffers with a final ionic strength of 150 m M (A: 25 m M glycine, 47.0 m M NaCl (pH 1.1); B) 25 m M sodium phosphate, 25 m M sodium acetate, 99.7 m M NaCl (pH 3.6); and C) 25 m M sodium phosphate, 83.5 m M NaCl (pH 7.5)) were mixed in a 9 1 ratio with ThT (1 m M in water).

Final samples were prepared by diluting the RNA and/or protein samples (U1A, U2B″, U2A′, or a mixture of these) with equal volumes of the final buffer supplemented with a final BME concentration of 5 mM.

Samples with 18 μM protein concentration were prepared at pH 7 and 8 in NaCl/phosphate buffer solution with a final ionic strength of 250 mM.

The pellets were suspended in buffer B with a final sucrose concentration of 2.2 mol/l and centrifuged at 100 000 × g for 1 h.

Following UV crosslinking, membranes were prehybridized in a rotating oven for 30 minutes at 42°C using ULTRAhyb-Oligo Hybridization Buffer (Ambion), followed by overnight hybridization in the same buffer at 42°C with a final concentration of 0.1 nM digoxigenin (DIG -labeled antisense miRCURY LNA probe (Exiqon).