Sentence examples for buffer with a concentration from inspiring English sources

GSH was dissolved in the same PBS buffer with a concentration from 0 to 80 mM.

Substrate stocks were prepared in the same assay buffer with a concentration of 200 μM cellobiose, xylobiose, or cellobionic acid.

Hepes Buffer with a concentration of 5 mmol/l at a pH of 7.4 was used in addition to the Krebs solution during the SNP experiments.

Each 10-μl PCR contained approximately 10 ng of DNA and 0.25 units of Taq DNA polymerase (Bioline) in the manufacturer's buffer with a concentration of 1.0 μM of each primer, 2.0 μM MgCl2 and 0.20 mM of each dNTP.

Molecular weight standards of albumin, carbonic anhydrase, cytochrome C and aprotinin (Sigma, St Louis, MO, USA) were prepared in 10 mM Tris HCl pH 7.4 and 150 mM NaCl buffer with a concentration of 3 mg/ml for aprotinin and carbonic anhydrase and a concentration of 2 mg/ml for albumin and cytochrome C. The column was calibrated with each standard at a flow rate of 0.3 ml/min.

The highest activity of rHLZ was at pH 6 when the salt concentration of the buffer was 0.1 M. When using a buffer with a salt concentration of 0.05 M, rHLZ showed lytic activity across a broader range of pH values (pH 5 9) than salt concentration of buffer was 0.1 M. The lytic activity of rHLZ declined sharply at extreme pH values in both ionic-strength buffers.

Bacteria suspended in the isotonic buffer solution with a concentration of 1 × 107 colony-forming units (CFU /ml and human blood cells were spiked into the prepared bacteria solution in a ratio of 1 400, giving a final blood cell concentration of 107 cells/ml.

The fd-dispersion is dialyzed against a TRIS/HCl buffer solution with a concentration of 0.032 mM, with a pH of 5.8.

To obtain the calibration curve of KA, we prepared a stock solution of 50 mL of a phosphate buffer solution with a concentration of 100  μg KA/mL in a volumetric flask.

The cells were later centrifuged to remove the citrate buffer and resuspended with PBS buffer with a cell concentration of 1 × 106 cells/mL.

Cells were thoroughly washed with 100 mM phosphate buffer (pH 7.0), and resuspended in the same buffer with a cell concentration of 100 kg/m3 and used for the biotransformation reaction.