Sentence examples for buffer with a cell from inspiring English sources

The cells were later centrifuged to remove the citrate buffer and resuspended with PBS buffer with a cell concentration of 1 × 106 cells/mL.

Cells were thoroughly washed with 100 mM phosphate buffer (pH 7.0), and resuspended in the same buffer with a cell concentration of 100 kg/m3 and used for the biotransformation reaction.

The washed platelets were then centrifuged again at 1000 × g for 10 min and finally re-suspended in Tyrode buffer with a cell density of 3×108 cells/ml.

The cells were then washed with DPBS, collected into RIPA lysis buffer with a cell scraper, and incubated on ice for 30 60 min.

Cells were then washed with DPBS, collected into RIPA lysis buffer with a cell scraper, and incubated on ice for 30 – 60 min.

After treatment, cells were washed with PBS, resuspended in kit lysis buffer and harvested with a cell scraper.

After rinsing with buffer A, cells were incubated with lysis buffer (1% nonidet P-40, 4 mg/ml dodecyl-β-D-maltoside, 0.8 mg/ml cholesteryl hemisuccinate in buffer A) plus protease inhibitors for one hour on ice.

The contents of each well were transferred simultaneously to the filter plate and washed 3−4 times with assay buffer using a cell harvester (Brandel Cell Harvester, Brandel Inc., Gaithersburg, MD).

After centrifuging and washing with buffer A, cells were suspended in 500  μL of buffer A and incubated in the dark for 15 min with 0.05 mM dihydroethidine (DHE).

After washing with buffer A, cells were incubated for 30 min at 37 °C with the ligation solution (Duolink II Ligation stock 1 : 5 and Duolink II Ligase 1 : 40).

The cells were left for 30 min on ice in this lysis buffer and then collected with a cell scraper.