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To amplify the canine and human BRCA2 promoter region, each reaction mixture contained 10-50 ng of genomic DNA as a template, each forward and reverse primer (Additional file 3: Table S2) at 300 nM, 200 μM dNTPs, 0.02 U of KOD FX DNA polymerase (Toyobo, Japan), and 1x PCR buffer, which was supplied with the enzyme, in a total volume of 10 μl.
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All diet ingredients were supplied by Harlan Teklad except BPA, which was supplied by NTP National Toxicology Programm, Durham NC).
His home village is in a buffer zone controlled by a Christian-run Lebanese militia, the South Lebanon Army, which is supplied by Israel.
To date, Buffer, which was first incubated in AngelPad, has raised some $400,000.
In ultra-deep submicron VLSI designs, clock network size must be minimized to reduce power consumption, power supply noise, and the number of clock buffers which are vulnerable to process variations.
Placebo (0.5 mL of sucrose-phosphate buffer) was supplied and administered using identical intranasal applicators.
Air was supplied to the batch flasks, which were filled with 50 mM Tris HCl buffer (pH 7.0) and placed in a water bath kept at 30 °C.
However, for practical purposes, the buffer can be supplied with the biosensor.
Note: Buffer RPE is supplied as a concentrate.
Cacodylate buffer and CoCl2 were supplied with TdT by Promega.
Both washing buffer and fixative were supplied with the kit.
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CEO of Professional Science Editing for Scientists @ prosciediting.com