Sentence examples for buffer which was prepared from inspiring English sources

The number of adhered cancer cells in the cultures was calculated from the calibration curves prepared by measuring intensity in the buffer which was prepared by lysing stained cancer cells at different concentrations.

Then 5 μl annexin V FITC was added to 195 μl of the cell suspension binding buffer, which was prepared by diluting the binding buffer 1 4 in distilled water (50 ml binding buffer +150 ml distilled water).

To improve the adhesive strength of the films, a compositional gradient B C N buffer layer, which was prepared by step-wise deposition with an increasing nitrogen flow rate from 0 sccm to 4.5 sccm in Ar/N2 mixed gas with a constant Ar flow rate of 25 sccm, was adopted before the nucleation stage of the cBN phase.

The assay mixture consisted of 1 ml of test solution, 2.9 ml of phosphate buffer (pH 7.5), and 0.1 ml of enzyme solution (0.01 units/ml in phosphate buffer, pH 7.5), which was prepared immediately before use.

In the assay, 2, 2-azobis (2-amidinopropane) dihydrochloride (AAPH) was dissolved in 10 ml of 75 mM phosphate buffer (pH 7.4) which was prepared daily as peroxyl-radical generator.

Partially purified α-amylase and amyloglucosidase (30.0 ml) was amalgamated in 2.5 liters of pre-gelatinized cassava starch slurry (20.0 gL-1) which was prepared in citrate buffer (pH-5.0, 50.0 mM).

Samples were derivatised using 6-aminoquinolyl N-hydroxysuccinimidyl carbamate which was prepared according to Cohen and Michaud [ 59], and buffered using 0.2 M borate buffer (sodium borate, sodium carbonate and sodium bicarbonate) pH 8.8.

Cells were stained with fluorescent dyes diluted in DMEM, except for annexin V and PI, which were prepared in a commercial binding buffer (Trevigen).

Kidney sections were fixed in 4% buffered paraformaldehyde, embedded in paraffin, and cut into 4-μm-thick sections which were prepared for HE and Masson staining.

A standard curve was generated by preparing a stock of 10 mM NaHPO4 in assay buffer which was stored at -20°C when not in use.

To exclude the latter possibility, we prepared extracts in SDS-PAGE sample buffer which is expected to solubilize all cellular proteins.