Sentence examples for buffer were washed with from inspiring English sources

Splenocytes in the buffer were washed with 1x ACK lysis buffer (Invitrogen), vortexed with a shaker, and centrifuged at 800 xg.

After stopping digestion with a serum- and calcium-containing buffer, cells were washed with PBS and collected by centrifugation (180 rcf, 1 min).

After incubation in collagenase-free buffer, cells were washed with PBS and then fixed in 100% methanol for 10 min at room temperature.

After 20 min fixation using the BD Cytofix/Cytoperm buffer, cells were washed with and stained in 0.5% w/v Saponin (Sigma) for 20 min on ice.

GST-CSL was further purified by binding to 10 mL of glutathione sepharose resin (GE Healthcare) pre-equilibrated with buffer 1. Beads were washed with buffer 1 and the GST fused CSL was eluted with 20 mM glutathione in buffer 1.

After quenching excess biotin with 100 mM quenching buffer, the cells were washed with TBS, and dissolved in 0.8 ml RIPA buffer to create whole cell lysates.

Staining buffer was removed and cells were washed with buffer.

The columns were washed with buffer PB and buffer PE.

Then the samples were washed with buffer containing phosphate-buffered saline and 0.5% bovine albumin.

Beads were washed with lysis buffer, boiled in Laemmli buffer, and subjected to SDS-PAGE.

Finally, they were washed with buffer solution to remove the unbound protein and excess GA.