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The pH adjustment by using 1.0 M HCl and 0.1 M NaOH for Zn II) at pH range 2.0 6.0, while 1.0 M HCl and ammonia buffer were used for Cd II) where the pH ranged from 2.0 to 8.0.
A microwave oven and citrate buffer were used for antigen retrieval [31].
In addition, note that the same elution profiles were obtained regardless of whether a sodium phosphate or Tris/HCl buffer were used for elution and irrespective of the NaCl concentration.
100 volumes of dialysis buffer were used for each step.
Native sample buffer and native running buffer were used for nondenaturing electrophoresis.
Bound immune complexes in radioimmunoprecipitation assay lysis buffer were used for immunoblotting after SDS-PAGE and NT transfer (9).
Similar(50)
Sodium acetate buffer was used for pH 4 6; Sodium-phosphate buffer was used for pH from 6 to 7 and Tris HCl buffer for pH 7 10.
Phosphate buffer was used for experiments at pH 7.0 and 8.0, and acetic acid buffer was used for those at pH 4.3 and 5.3.
After that, FDA and PI working buffer was used for cell staining (Calcein AM/Ethidium Homodimer, Invitrogen).
Agarose 0.8%% (w/v) in 0.5X TAE (pH 8.0) buffer was used for submarine gel electrophoresis.
Native alpha chymotrypsin (1 mg mL-1) solution in the aqueous buffer was used for recording the spectrum.
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buffer were applied for
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buffer were recorded for
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buffer were run for
buffer were amplified for
buffer were microwaved for
buffer were collected for
buffer were averaged for
buffer were centrifuged for
buffer were performed for
buffer were sonicated for
buffer were boiled for
buffer were incubated for
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