Sentence examples for buffer were treated with from inspiring English sources

300 µg antibodies in 300 µl PBS supplemented with G7 reaction buffer were treated with 10 units/µl PNGase F (New England Biolabs) for 6 hrs to deglycosylate the antibodies.

The cells in arginine-containing buffer, arginine-free buffer, and rADI pretreated arginine-containing buffer were treated with NMDA for 1 h.

For deglycosylation reactions, samples denatured in protein loading buffer were treated with 500 units of PNGase F (NEB) in 1× G7 Reaction Buffer and 1% NP40 for 1.5 hours at 37°C.

Cells (1 × 10/ml stain buffer) were treated with 5% normal mouse serum (Sigma-Aldrich, St . Louis MO, USA) for 10 min prior to staining with 7-AAD and the antibody cocktail mentioned above.

One third of the lysate in Passive Lysis Buffer was treated with 200 µl Trizol reagent and chloroform to extract RNA for reverse transcription (RT -PCR.

250 nl 10% ADBH diluted into 0.5x capture buffer was treated with or without 3 M guanidine thiocyanate for 10 minutes at 95°C and then centrifuged for 1.5 hour at 134,000 g at 4°C.

In the case of the initial assessment of the inhibitory power of the LF (data in Figure  1), the LF (with the pH adjusted to pH 5 by the addition of 0.5 M sodium acetate buffer) was treated with 0.1 μM N188BG for 48 h before the inhibition studies.

Briefly, 40 μL of the TTR variants at 4 μM (in GF buffer) was treated with 4 μL of glutaraldehyde (25% solution, Sigma) and incubated at room temperature for 5 min. The reactions were quenched by addition of 4 μL of 7% NaBH4 (freshly prepared in 0.1 M NaOH).

For multiplex analysis detection, the nanocomplex of 1 nM in PBS buffer was treated with the Tubb3 targets (single-base mismatched or complementary DNA targets) or Fox3 targets (single-base mismatched or complementary DNA targets) at various concentrations of 0, 2, 5, 10, 25, 50, 100, and 200 nM.

At first, nanodiamond precipitates containing the adsorbed or immobilized IgGI125 preliminarily washed with PBS-buffer were treated with serum as follows.

Apo-S100B experiments used EDTA-treated protein, and all buffers were treated with Chelex resin (BioRad, ON, Canada) to remove any free calcium.