Sentence examples for buffer were then transferred from inspiring English sources

The membrane tray was then placed in a new lower chamber containing pre-warmed detachment buffer (Accutase, Sigma-Aldrich, St . Louis MO) at 37°C for 10 min. Detached cells in the buffer were then transferred to a V-bottom 96 well plate and centrifuged at 1500 rpm for 5 min, buffer aspirated and froze at −20°C overnight.

The haustoria in the suspension buffer were then transferred to a 15 mL Eppendorf tube and centrifuged for 1 min at 10,000 g and the pellet frozen in liquid nitrogen and stored at -80°C.

Total RNA from pooled section scrapes corresponding to each cap was prepared by pipeting 50 μL of extraction buffer (Picopure RNA Isolation kit) directly onto the tissue section on the glass slide, using the pipette tip to gently scrape the tissue into the buffer Samples were then transferred into an RNase-free microcentrifuge tube and stored at -80°C.

Gels were run in NuPAGE MOPS SDS running buffer, and protein bands were then transferred to a polyvinylidene difluoride (PVDF) membrane.

The specimens were then transferred to buffer and processed routinely for embedding in Paraplast® (Merck, Darmstadt, Germany).

The samples were then transferred to Laemmli buffer, and the proteins were separated on a 12.5% SDS-PAGE and were visualized by Commassie-R250-staining.

Proteins were then transferred in Towbin buffer onto a PVDF membrane and probed with specific antibodies (Diagenode rabbit anti-H2A.Zac or Diagenode rabbit anti-H3K9ac [Diagenode, Liège, Belgium]).

F1 animals were then transferred into lysis buffer in indexed PCR tubes and were screened using primers outside the homology arms followed by restriction enzyme digestion to detect the insertion.

Sections were then transferred into a lysis buffer.

The embryos were then transferred to post-transplantation buffer (0.1× MMR, 5 μg/ml gentamicin) and reared until stage 32, when they were fixed for analysis.

They were then transferred to 30% sucrose in phosphate buffer (PB) and left overnight.