Sentence examples for buffer were then separated from inspiring English sources

Beads and buffer were then separated by magnetic separation and the supernatant was removed.

Beads and buffer were then separated by magnetic separation and the buffer was moved to a new 1.5 mL microfuge tube.

Equal amounts of protein (10 μg) from each sample, and 2 μg of biotinylated broad range protein molecular weight markers (Bio-Rad, Hercules, CA) were treated with a reducing sample buffer; samples were then separated on a 12.5% SDS-PAGE minigel, and electroblotted onto 0.2-μm nitrocellulose membranes (Amersham) (100 V, 500 mA, 4°C, 40 min).

The bead-bound cells were then separated by magnetic separation and lysed immediately in Trizol buffer (Invitrogen) for RNA isolation.

Supernatants were mixed with sodium dodecyl sulfate-polyacrylamidegel electrophoresis (SDS-PAGE) sample buffer and boiled for 10 min, and were then separated on 10% SDS PAGE gel.

When prepared under non-denaturing conditions, samples were first mixed with 2X SDS-PAGE loading buffer and boiled for 5 min. Proteins were then separated on 12% polyacrylamide gels and detected by silver nitrate staining or transferred to a PVDF membrane for immunoblotting.

The protein pellet was subsequently resuspended in 2X SDS-protein reducing buffer and boiled for 10 min. Proteins were then separated by SDS-PAGE analyzedyzed by in-gel fluorescent scanning using a Typhoon scanner (GE Healthcare; excitation at 532 nM, emission at 580 nM).

LDs (LD) were then separated from the under-layer buffer (UB) and both were processed for SDS-PAGE.

PCR products were then separated by electrophoresis in a 1.5% agarose gel in TAE buffer (40 mM Tris acetate, 2 mM EDTA) containing GelRed (Biotium, Hayward, CA, USA).

Proteins were then separated by SDS-PAGE.

Several replicates were then separated on 12% pre-cast NuPAGE Novex gels (Invitrogen, Carlsbad, CA, USA) using a 2- N-morpholine -ethanesulphonic acid (MES) buffer system.