Sentence examples similar to buffer were then removed from inspiring English sources

The buffer was then removed, and the slides were placed into staining jars containing radioligand solution (concentration range of 0.1 10 nM) in the incubation buffer with or without 10 μM raclopride (D2/3 antagonist) or 100 μM guanosine-5'-triphosphate sodium salt (GTP, stimulator of G-protein uncoupling from the receptors).

The lower running buffer was then removed and the lower chamber rinsed with nuclease-free water 2 times.

The lower running buffer was then removed and the RNA was purified using the flashPAGE™ clean up kit, producing RNA fragments ranging from ∼50 150 nt in size.

Excess RIPA buffer was then removed from the beads.

The SAPE/Amplification Buffer was then removed by vacuum filtration.

Dye loading buffer was then removed and replaced by pre-warmed complete growth media.

The Triton/Ellman buffer was then removed from the vial and analyzed for cholinesterase activity.

Buffer was then removed from each well, cells washed once in HBSS and fresh HBSS added at room temperature.

The buffer was then removed and samples were incubated in reaction buffer and TdT enzyme mix at 37°C for 90 min.

Chelation buffer was then removed and 5 ml of shaking buffer (PBS with 43.3 mM sucrose and 54.9 mM sorbitol) was added.

To obtain SDS lysates cells were washed three times with PBS at room temperature, residual wash buffer was then removed and cells were scraped with boiling SDS-PAGE sample buffer, followed by 5 min boiling in a hot block.