Sentence examples for buffer were then mixed from inspiring English sources

Twenty-seven microfiters of GenomiPhi Reaction buffer were then mixed with 3 μl GenomiPhi enzyme, and 25 μl of this mix were added to the denatured genomic DNA.

The three stock solutions (silica NPs, protein, and buffer) were then mixed in known proportions to arrive at eight different protein concentrations (0.5 5 mg/mL), three different NaCl concentrations (0, 25, and 100 mM), and a constant mass fraction of Ludox TMA (about 1 wt%).

The samples were then mixed with the loading buffer without a reducing agent.

Dye coupled cDNAs were then mixed together in a hybridization buffer consisting of 0.25% SDS, 25 mM HEPES, and 3×SSC.

Samples were then mixed with 2% SDS loading buffer of for 20 min. Their molecular weights were analyzed on a 3 12% Tris-Glycine gel in SDS running buffer.

The samples were then mixed with a chaotropic salt binding buffer and 100% ethanol for binding to silica filters.

Washed beads were then mixed with 30 µl 2× SDS-PAGE buffer and analyzed on a 10% polyacrylamide gel.

The samples were then mixed with 60 μl of pre-warmed hybridization buffer.

Eight parts of collagen were then mixed with one part 10 × Hank's Buffered Salt Solution (Sigma-Aldrich Company Ltd., Poole, Dorset, UK).

Reactions were then mixed by pipetting and loaded directly into wells of a gel comprising 7% polyacrylamide in 1× TBE buffer.

The labeled samples were then mixed together.