Sentence examples for buffer were then exposed from inspiring English sources

Worms in L1 diapause suspended in liquid buffer were then exposed to an acute heat stress at 36.8° in a shaking incubator (70 rpm) for 4 hr in a sealed microcentrifuge tube.

Membranes were washed under stringent conditions (45 min at 65°C in 2× sodium citrate buffer, SSC) and were then exposed to an imaging plate for quantification of radioactivity by a BAS-1500 Phosphoimager (Fuji, Nakanuma, Japan).

Sections were then exposed to citrate buffer using a carousel microwave at 50 W for 15 min and then Dako REAL™ Peroxidase Blocking Solution at room temperature for 30 min.

Clone 9 cells after treatment were then exposed to phosphate buffered saline (PBS) free medium containing 250 μM H2O2 for 1 hr.

The reactions were stopped by the addition of one volume of loading buffer, fractionated on denaturing 5 8% PAGE gels which were then exposed to PhosphorImager screens.

Cells were then exposed to the precipitate for 16 h, washed twice in phosphate buffered saline (PBS), and then incubated with DMEM F12 containing 10% fetal bovine serum.

Tubes were then exposed to 10 transforming units of the phage library in 2% MPBS, 0.1% Tween-20 buffer for 2 h.

Cell were then exposed to an antibody directed against POU5F1 (1 200, Santa Cruz Biotechnology, catalog no. sc-9081) or to IgG (0.4 μg/mL; Santa Cruz Biotechnology, catalog no. sc-66931) in the blocking buffer for 1 h.

The embryos were then exposed.

Membranes were then exposed to KODAK X-ray film.

Mitochondria were then exposed to different chemicals.