Sentence examples for buffer were then applied from inspiring English sources

Slides with metaphase spreads were treated with 70% deionized formamide in 2 × SSC at 70°C for 2 min. Denatured probes in hybridization buffer were then applied to the slides, which were incubated at 37°C for 10 h in a humid chamber.

Equilibration Buffer was then applied on the sections for 3 min and finally, they were incubated with Working Strength TdT Enzyme for 1 h at 37°C in a humid chamber.

The supernatants were removed by centrifugation, and the pellets were boiled in 2× sample buffer for 4 min. The products were then applied to sodium dodecyl sulphate (SDS)–polyacrylamide gel electrophoresis (PAGE).

A 0.5 mL sample was applied to a HiTrap Desalting, equilibrated with 0.02 M citrate-phosphate buffer (pH 4.0), and fractions at the first peak were then applied to a HiTrap DEAE FF.

The protein solution was concentrated with ultrafiltration by Amicon filters (100-kDa cutoff) to 4 5 ml, which were then applied to a Sephacryl S-400 HR column (2.6 × 60 cm) equilibrated with buffer B (buffer A + 150 mM NaCl).

The dialyzed, pooled fractions were then applied to an SP-Sepharose FF column (2 × 20 cm) equilibrated with 0.02 M citrate-phosphate buffer (pH 4.0).

Watershed and skeletonization filters were then applied.

Nonparametrical analyses were then applied.

Additional filters were then applied.

Secondary fluorescein isothiocyanate (FITC -conjugated anti-mouse antibody (diluted 1:200 in blocking buFITC -conjugatedmmunoResearch Laboratories) wantibodyappliediluted h.

After the column was washed with 240 mL buffer A, the enzyme was eluted with 0.3 M NaCl in buffer A. The enzyme solution was then applied to a heparin-Sepharose column (1.6 cm × 15 cm), which was preequilibrated with buffer A containing 0.2 M NaCl.