Sentence examples for buffer were then added from inspiring English sources

Serial dilutions of Rad51 in blocking buffer were then added to the appropriate wells of the ELISA plate and incubated for 1 h at 30°C.

50 µl of beads (in binding/wash buffer) were then added to RNA, incubated at 42°C for 20 min, and then shaken for 2 h at room temperature.

The appropriate antibody mixes or single stains in the optimized concentrations (diluted in FACS buffer) were then added onto the Fc blocked cells in additional 50 µl and incubated for 30 minutes on ice in dark conditions.

105 cells in 100 µl migration buffer were then added to the top of each migration chamber and allowed to migrate to the underside of the porous membrane for various times in triplicate.

RNase Inhibitor, cDNA enzyme mix, and 5× synthesis buffer were then added and the mixture heated at 42°C for 50 minutes, followed by 5 minutes heat inactivation at 75°C.

Five mL of dilution buffer were then added and the mixture homogeneized and centrifuged.

The solution was removed after 30 s, the mica surface was washed three times with 40 μL TE buffer containing 10 mM MgCl2 and 100 mM NaCl, and 80 μL of 1 × TE/Mg2+ buffer was then added before imaging.

The samples were further incubated at 80 °C for 2 h and then centrifuged for 20 min at 16,000× g. 6x SDS sample buffer was then added to the supernatant and samples were then boiled again for 10 min and loaded onto the gel.

Buffer is then added by hand before transferring the vial to a heating block with a forceps.

Approximately 10 MBq of 111In in acetate buffer was then added to the conjugated AbD15179, and the reaction mixture was allowed to incubate for 30 min at room temperature.

400 µl of 2% (w/v) azocasein in activation buffer was then added and incubated for a further 1 h at 40°C.