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Antibody samples serially diluted in running buffer were tested in duplicate with the final data sets collated from two separate experiments.
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The efficiency of iron removal from Lf in citrate buffer was tested in a pH range of 2 5 (Fig. 4).
These buffers were tested in different molarities for their capacity to elute rbST from the monolith micro-columns.
Enzymes peroxidase, lactoperoxidase and alkaline phosphatase in phosphate buffer were tested and activity was determined with commercial reflectometric strips.
Therefore, various volumes of the 1 µl bloodstain (from a 25 µl extract in Tris buffer) were tested ranging from 0.25 3 µl (equivalent to 10 120 nl of liquid blood).
Following that, in the study of temperature effect, bicarbonate and borate buffer were tested to find out which buffer is more appropriate.
Two treatments (with and without a cornstalk buffer strip) were tested in the following four runs: 1) without cornstalk buffer strip, 2) with cornstalk buffer strip in the third rain event, 3) with cornstalk buffer strip in the second rain event, 4) with continuous cornstalk buffer strip in all three successive rainfall events.
However, all buffers used were tested in a control group of chicken embryos (PBS control) in each individual experiment.
The native PHS and PHS nanogel stabilizing against thermal denaturation were tested in acetate buffer (100 mM, pH 4.5) at 60 °C and the activity was determined after sampling periodically as described above.
The cargo-loaded vesicles were tested in PBS buffer with different glucose concentrations, including a typical hyperglycemic level (400 mg/dL) and a normal level (100 mg/dL).
Therefore, Sorenson's phosphate buffers from pH 6.0 to 8.0 were tested in steps of 0.5 pH units.
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