Sentence examples for buffer were supplemented with from inspiring English sources

Lysis and wash buffer were supplemented with 5 mM imidazole and elution buffer supplemented with 300 mM imidazole.

For MNase fragmentation, nuclei in sort buffer were supplemented with PBS containing 0.1% Triton-X100 (PBS-Tx) to a volume of 400 µl, and CaCl2 to 1 mM.

As shown in Figure 4, an epitope tagged T7-IQD20 fusion protein preferentially co-sedimented with calmodulin-agarose beads in the presence of Ca2+, whereas noticeably less T7-IQD20 protein was bound to immobilized calmodulin when the incubation mix and wash buffer were supplemented with EGTA.

Whole cell extract (25 μg total protein), recombinant human legumain (5 ng; R&D Systems, MN, USA; 2199-CY) or pellets from TCA precipitation dissolved in RIPA lysis buffer were supplemented with Laemmli SDS-PAGE sample buffer (Invitrogen) and boiled at 100°C for 5 min. Samples were applied to 4-12% gradient gels in 2- N-morpholino) ethanesulfonic acid (MES) buffer for SDS-PAGE (Invitrogen).

For purification of the hydantoinase, the buffer was supplemented with 1 mM ZnSO4, while for the carbamoylase 5 mM DTT were added.

Lysis buffer was supplemented with Complete EDTA-free protease inhibitor cocktail (Roche).

The lysis buffer was supplemented with the Complete Protease Inhibitor Cocktail, EDTA-free (Roche).

For immunoprecipitation assays, this lysis buffer was supplemented with 10% glycerol.

To analyze co-immunoprecipitation of CSP and VAMP2, the lysis buffer was supplemented with 1% SDS.

Prior to use, the lysis buffer was supplemented with Protease- and Phosphatase-inhibitors (Pierce, Rockford, IL).

Lysis buffer was supplemented with protease and phosphatase inhibitors, including 1 mM sodium fluoride, 1 µg/ml leupeptin, 1 µg/ml aprotinin, and 1 mM PMSF (Phenylmethanesulfonyl fluoride).