Sentence examples for buffer were sonicated for from inspiring English sources

One hundred microliters of brain homogenate in HS buffer were sonicated for 5 min using a water-bath sonicator and then centrifuged at 100,000 × g for 30 min at 4 °C.

The resuspended cells in 500 μL of the same buffer were sonicated for five pulses of 30 s and pauses of 30 s on ice with a sonicator (Laballiance SL-600S, USA).

For control experiments in the absence of H2O2 and oxygen, 100 mL of the buffer was sonicated for 60 min, and residual oxygen was removed by purging with nitrogen.

The blood mixed with buffer was sonicated for 3 min, and its fluorescence measured with a fluorescence microplate reader (Tecan Japan Co., Ltd).

The cells or spinal cord lysates of the transgenic mice in TNE lysis buffer were sonicated and centrifuged at 15 000 × g for 5 min at 4 °C.

After addition, the buffer cells were sonicated for 30 s and centrifuged at 14 000 r.p.m. for 10 min and the supernatant was collected.

To determine the changes in the expression of Bcl-2 family proteins in TM40D cells, cells induced under different procedures were collected and homogenized in lysis buffer and were sonicated for 4 times for 5 seconds each on ice.

Cell paste in start buffer was sonicated on ice for 20 min. After centrifugation (23700 g for 30 min, Beckman JA-20 rotor), the supernatant was filtered through a 0.45 μm syringe filter.

After being incubated with the label for 30 min, the cells were rinsed in ice-cold PBS containing 10 mM methionine and scraped gently from the filter in 0.2 mL buffer A. The cells were sonicated for 10 seconds and centrifuged for 5 minutes at 13,000 g in a refrigerated microfuge.

50 µl 4× Laemmli buffer was added and the samples were sonicated for 7 min (30 s interval, power 'low') using a Bioruptor (Diagenode, Liège, Belgium) and centrifuged for 10 min at 4°C at maximum speed.

For UCP3 protein determination, muscle biopsies were homogenized in ice-cold Tris-EDTA buffer at pH 7.4, and then the homogenates were sonicated for 15 seconds.